Abstract
Extracellular matrix (ECM) molecules constitute a “niche” that modulates the migration, proliferation, and differentiation of neural stem/progenitor cells (NSPCs). The glycoprotein Tenascin-R (TN-R) is an ECM molecule, comprising multiple domains. Either the whole TN-R molecule or its distinct domains has been demonstrated to play a very important role in the developing central nervous system. However, little is known about the effect of the TN-R domain on NSPCs, especially NSPC migration. In the present study, we first show that both TN-R domains epidermal growth factor-like repeat (EGFL) and fibronectin type III (FN)6–8 can inhibit the NSPCs migration from neurospheres in vitro. Furthermore, both the EGFL and FN6–8 domains affect the distribution of neurons generated from neurospheres, indicating that EGFL and FN6–8 domains inhibit the motility of neurons generated from neurospheres. These results suggest that TN-R has an inhibitory effect on NSPCs migration.
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Acknowledgments
We thank Dr. Zhi-cheng Xiao and Prof. Melitta Schachner for providing some plasmid and reagents. This work was supported by grants from the National Natural Science Foundation of China (30670793), Natural Science Foundation of Jiang Su Province (BK2006150), International Coorperation Projects of Jiang Su Province (BZ2006050), Pharmacodynamics research and evaluation Fund of Jiang Su Province and Initial Fund of China Pharmaceutical University to Dr. Hong Liao.
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Editor: J. Denry Sato
Wenhui Huang and Luyong Zhang These author contributed equally to this work
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Huang, W., Zhang, L., Niu, R. et al. Tenascin-R distinct domains modulate migration of neural stem/progenitor cells in vitro. In Vitro Cell.Dev.Biol.-Animal 45, 10–14 (2009). https://doi.org/10.1007/s11626-008-9145-6
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DOI: https://doi.org/10.1007/s11626-008-9145-6