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The Treatment with Antibody of TNF-α Reduces the Inflammation, Necrosis and Fibrosis in the Non-alcoholic Steatohepatitis Induced by Methionine- and Choline-deficient Diet

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Abstract

To assess the effects of anti-TNF-α antibody (infliximab) in experimental steatohepatitis induced by methionine- and choline-deficient (MCD) diet. The study included thirty rats. One group received normal rat food, and two groups received MCD diet. The treatment group received a single dose intra-peritoneal infliximab (4 mg/kg), at week 8. MCD diet increased levels of AST, ALT, TNF-α, TGF-β1, tissue and plasma MDA (p < 0.05 for each). Moreover, it led to steatosis, ballooning degeneration, inflammation, fibrosis and increased actin expression, histopathologically (p < 0.05 for each). In this experimental steatohepatitis anti-TNF-α antibody decreased the levels of AST, ALT, TGF-β1 and plasma and tissue MDA (p < 0.05 for each). Moreover, inflammation, necrosis, actin expression and fibrosis decreased in anti-TNF-α group compared to placebo group (p < 0.05 for each). This study indicates that anti-TNF-α antibody is effective on necrosis, inflammation and fibrosis in the experimental model of non-alcoholic steatohepatitis, induced by MCD diet.

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Authors and Affiliations

  1. Faculty of Medicine, Department of Internal Medicine and Rheumatology, Fırat University, Elazig, Turkey

    Suleyman Serdar Koca

  2. Faculty of Medicine, Department of Gastroenterology, Fırat University, Elazig, Turkey

    Ibrahim Halil Bahcecioglu & Orhan Kursat Poyrazoglu

  3. Faculty of Medicine, Department of Pathology, Fırat University, Elazig, Turkey

    Ibrahim Hanifi Ozercan

  4. Faculty of Veterinary, Department of Animal Nutrition, Fırat University, Elazig, Turkey

    Kazim Sahin

  5. Faculty of Medicine, Department of Biochemistry, Fırat University, Elazig, Turkey

    Bilal Ustundag

  6. Tıp Fakultesi, Fırat universitesi, Romatoloji BD, 23119, Elazig, Turkey

    Suleyman Serdar Koca

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  1. Suleyman Serdar Koca
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Correspondence to Suleyman Serdar Koca.

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Koca, S.S., Bahcecioglu, I.H., Poyrazoglu, O.K. et al. The Treatment with Antibody of TNF-α Reduces the Inflammation, Necrosis and Fibrosis in the Non-alcoholic Steatohepatitis Induced by Methionine- and Choline-deficient Diet. Inflammation 31, 91–98 (2008). https://doi.org/10.1007/s10753-007-9053-z

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  • DOI: https://doi.org/10.1007/s10753-007-9053-z

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