Primer of mouse gene used for quantitative real-time PCR analysis
Gene name | Primers sequences |
SIRT1 | For:5-GCAACAGCATCTTGCCGAT-3; Rev:5-GTGCTACTGGTCTCACTT-3 |
CuZnSOD (superoxide dismutase) | For:5-GCGGTGAACCAGTTGTGTTGTC-3; Rev:3-CCTCTGGACCCGTTACACTGAC-5 |
MnSOD (superoxide dismutase) | For:5-ATGTTACAACTCAGGTCGCTCTTC-3; Rev:3-CCTCTCAACGACCTCCGATAGT-5 |
Catalase | For:5′-CCGACCAGGGCATCAAAA; Rev:5′-GAGGCCATAATCCGGATCTTC-3′ |
Glutathione peroxidase (GPx) | For:5′-ATGGGCATAAACTTGGGAGACATTTT-3′; Rev:5′-CCTAATTCTGTTGTACAAACAGGGGTATA-3′ |
p22phox | For:5-GCGGTGTGGACAGAAGTACC-3; Rev:5-CTTGGGTTTAGGCTCAATGG-3 |
Nox-4 | For:5-ACAGTCCTGGCTTACCTTCG-3; Rev:5-TTCTGGGATCCTCATTCTGG-3 |
Adipose triglyceride lipase (ATGL) | For:5′-GAGCCCCGGGTGGAACAAGAT-3′; Rev:5′-AAAAGGTGGTGGGCAGGAGTAAGG-3′ |
Hormone sensitive lipase (HSL) | For:5′-GCCGGTGACGCTGAAAGTGGT-3′; Rev:5′-CGCGCAGATGGGAGCAAGAGGT-3′ |
Monoglyceride lipase (MGL) | For:5′-CAGAGAGGCCCACCTACTTTT-3′; Rev:5′-ATGCGCCCCAAGGTCATATTT-3′ |
PPARα | For:5′-CCGAACATTGGTGTTCGCAG-3′; Rev:5′-AGATACGCCCAAATGCACCA-3′ |
Myogenic differentiation (MyoD) | For:5′-CCGCCTGAGCAAAGTAAATGA-3′; Rev:5′-GCAACCGCTGGTTTGGATT-3′ |
Muscle atrophy F-box (MAFbX) | For:5′-AGAAAAGCGGCACCTTCGT-3′; Rev:5′-CTTGGCTGCAACATCGTAGTT-3′ |
Murf-1 | For:5′-CCTACTTGCTCCTTGTGC3′; Rev:TCCTGCTC CTGC GTGAT-3′ |
Myosin heavy chain (MCH-II) | For:5′-AAGGTCGGCAATGAGTATGTCA-3′; Rev:5′-CAACCATCCACAGGACACTCTTC-3′ |
LC3-II | For:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3 |
Beclin-1 | For:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3 |
Atg5 | For:5′-AGATGGACAGCTGCACACAC-3′; Rev:5′-GCTGGGGGACAATGCTAATA-3′ |
Atg 7 | For:5′-TCCGTTGAAGTCTCTGCTT-3′; Rev:5′-CCACTGAGGTTCACCATCCT-3′ |
Caspase-3 | For:5′-GGTATTGAGACAGACAGTGG-3′; Rev:5′-CATGGGATCTGTTTCTTTGC-3′ |
Caspase-9 | For:5′-AGTTCCCGGGTGCTGTCTAT-3′; Rev:5′-GCCATGGTCTTTCTGCTCAC-3′ |
18S | For:5′-GTAACCCGTTGAACCCCATT-3′; Rev:5′-CCATCCAATCGGTAGTAGCG-3′ |
PPAR, peroxisome proliferator-activated receptor.