Table 1

Primer of mouse gene used for quantitative real-time PCR analysis

Gene namePrimers sequences
SIRT1For:5-GCAACAGCATCTTGCCGAT-3; Rev:5-GTGCTACTGGTCTCACTT-3
CuZnSOD (superoxide dismutase)For:5-GCGGTGAACCAGTTGTGTTGTC-3; Rev:3-CCTCTGGACCCGTTACACTGAC-5
MnSOD (superoxide dismutase)For:5-ATGTTACAACTCAGGTCGCTCTTC-3; Rev:3-CCTCTCAACGACCTCCGATAGT-5
CatalaseFor:5′-CCGACCAGGGCATCAAAA; Rev:5′-GAGGCCATAATCCGGATCTTC-3′
Glutathione peroxidase (GPx)For:5′-ATGGGCATAAACTTGGGAGACATTTT-3′; Rev:5′-CCTAATTCTGTTGTACAAACAGGGGTATA-3′
p22phoxFor:5-GCGGTGTGGACAGAAGTACC-3; Rev:5-CTTGGGTTTAGGCTCAATGG-3
Nox-4For:5-ACAGTCCTGGCTTACCTTCG-3; Rev:5-TTCTGGGATCCTCATTCTGG-3
Adipose triglyceride lipase (ATGL)For:5′-GAGCCCCGGGTGGAACAAGAT-3′; Rev:5′-AAAAGGTGGTGGGCAGGAGTAAGG-3′
Hormone sensitive lipase (HSL)For:5′-GCCGGTGACGCTGAAAGTGGT-3′; Rev:5′-CGCGCAGATGGGAGCAAGAGGT-3′
Monoglyceride lipase (MGL)For:5′-CAGAGAGGCCCACCTACTTTT-3′; Rev:5′-ATGCGCCCCAAGGTCATATTT-3′
PPARαFor:5′-CCGAACATTGGTGTTCGCAG-3′; Rev:5′-AGATACGCCCAAATGCACCA-3′
Myogenic differentiation (MyoD)For:5′-CCGCCTGAGCAAAGTAAATGA-3′; Rev:5′-GCAACCGCTGGTTTGGATT-3′
Muscle atrophy F-box (MAFbX)For:5′-AGAAAAGCGGCACCTTCGT-3′; Rev:5′-CTTGGCTGCAACATCGTAGTT-3′
Murf-1For:5′-CCTACTTGCTCCTTGTGC3′; Rev:TCCTGCTC CTGC GTGAT-3′
Myosin heavy chain (MCH-II)For:5′-AAGGTCGGCAATGAGTATGTCA-3′; Rev:5′-CAACCATCCACAGGACACTCTTC-3′
LC3-IIFor:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3
Beclin-1For:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3
Atg5For:5′-AGATGGACAGCTGCACACAC-3′; Rev:5′-GCTGGGGGACAATGCTAATA-3′
Atg 7For:5′-TCCGTTGAAGTCTCTGCTT-3′; Rev:5′-CCACTGAGGTTCACCATCCT-3′
Caspase-3For:5′-GGTATTGAGACAGACAGTGG-3′; Rev:5′-CATGGGATCTGTTTCTTTGC-3′
Caspase-9For:5′-AGTTCCCGGGTGCTGTCTAT-3′; Rev:5′-GCCATGGTCTTTCTGCTCAC-3′
18SFor:5′-GTAACCCGTTGAACCCCATT-3′; Rev:5′-CCATCCAATCGGTAGTAGCG-3′
  • PPAR, peroxisome proliferator-activated receptor.