Primer of mouse gene used for quantitative real-time PCR analysis
| Gene name | Primers sequences |
| SIRT1 | For:5-GCAACAGCATCTTGCCGAT-3; Rev:5-GTGCTACTGGTCTCACTT-3 |
| CuZnSOD (superoxide dismutase) | For:5-GCGGTGAACCAGTTGTGTTGTC-3; Rev:3-CCTCTGGACCCGTTACACTGAC-5 |
| MnSOD (superoxide dismutase) | For:5-ATGTTACAACTCAGGTCGCTCTTC-3; Rev:3-CCTCTCAACGACCTCCGATAGT-5 |
| Catalase | For:5′-CCGACCAGGGCATCAAAA; Rev:5′-GAGGCCATAATCCGGATCTTC-3′ |
| Glutathione peroxidase (GPx) | For:5′-ATGGGCATAAACTTGGGAGACATTTT-3′; Rev:5′-CCTAATTCTGTTGTACAAACAGGGGTATA-3′ |
| p22phox | For:5-GCGGTGTGGACAGAAGTACC-3; Rev:5-CTTGGGTTTAGGCTCAATGG-3 |
| Nox-4 | For:5-ACAGTCCTGGCTTACCTTCG-3; Rev:5-TTCTGGGATCCTCATTCTGG-3 |
| Adipose triglyceride lipase (ATGL) | For:5′-GAGCCCCGGGTGGAACAAGAT-3′; Rev:5′-AAAAGGTGGTGGGCAGGAGTAAGG-3′ |
| Hormone sensitive lipase (HSL) | For:5′-GCCGGTGACGCTGAAAGTGGT-3′; Rev:5′-CGCGCAGATGGGAGCAAGAGGT-3′ |
| Monoglyceride lipase (MGL) | For:5′-CAGAGAGGCCCACCTACTTTT-3′; Rev:5′-ATGCGCCCCAAGGTCATATTT-3′ |
| PPARα | For:5′-CCGAACATTGGTGTTCGCAG-3′; Rev:5′-AGATACGCCCAAATGCACCA-3′ |
| Myogenic differentiation (MyoD) | For:5′-CCGCCTGAGCAAAGTAAATGA-3′; Rev:5′-GCAACCGCTGGTTTGGATT-3′ |
| Muscle atrophy F-box (MAFbX) | For:5′-AGAAAAGCGGCACCTTCGT-3′; Rev:5′-CTTGGCTGCAACATCGTAGTT-3′ |
| Murf-1 | For:5′-CCTACTTGCTCCTTGTGC3′; Rev:TCCTGCTC CTGC GTGAT-3′ |
| Myosin heavy chain (MCH-II) | For:5′-AAGGTCGGCAATGAGTATGTCA-3′; Rev:5′-CAACCATCCACAGGACACTCTTC-3′ |
| LC3-II | For:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3 |
| Beclin-1 | For:5-AATCTAAGGAGTTGCCGTTATAC-3; Rev:5-CCAGTGTCTTCAATCTTGCC-3 |
| Atg5 | For:5′-AGATGGACAGCTGCACACAC-3′; Rev:5′-GCTGGGGGACAATGCTAATA-3′ |
| Atg 7 | For:5′-TCCGTTGAAGTCTCTGCTT-3′; Rev:5′-CCACTGAGGTTCACCATCCT-3′ |
| Caspase-3 | For:5′-GGTATTGAGACAGACAGTGG-3′; Rev:5′-CATGGGATCTGTTTCTTTGC-3′ |
| Caspase-9 | For:5′-AGTTCCCGGGTGCTGTCTAT-3′; Rev:5′-GCCATGGTCTTTCTGCTCAC-3′ |
| 18S | For:5′-GTAACCCGTTGAACCCCATT-3′; Rev:5′-CCATCCAATCGGTAGTAGCG-3′ |
PPAR, peroxisome proliferator-activated receptor.