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Circulating microRNAs as potential biomarkers to detect transformation of Barrett’s oesophagus to oesophageal adenocarcinoma
  1. Juntaro Matsuzaki1,2,
  2. Hidekazu Suzuki3
  1. 1 Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan
  2. 2 Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan
  3. 3 Medical Education Center, Keio University School of Medicine, Tokyo, Japan
  1. Correspondence to Dr Hidekazu Suzuki; hsuzuki{at}


Objective Circulating microRNAs (miRNAs) are promising biomarkers for the early detection of cancers. This study aimed to address potential circulating miRNAs to monitor the progression from Barrett’s oesophagus (BO) to oesophageal adenocarcinoma (OAC).

Design We comprehensively analysed tissue and serum miRNA expression profiles of BO mice model (L2-interleukin-1β (IL-1β) mice) using microarray analysis. To validate the data from mice, a published dataset of human plasma miRNAs, consisting of eight patients with OAC, eight with BO and six healthy controls, was used (GSE51410).

Results We identified 20 upregulated miRNAs and 44 downregulated miRNAs both in tissues and in sera of 46-week-old mice compared with 28-week-old mice. Two of the 20 miRNAs (miR-128-3 p and miR-328-3 p) were upregulated, and five of the 44 miRNAs (miR-143-3 p, miR-144-3 p, miR-15a-5p, miR-1-3 p and miR-133b) were downregulated in plasma of patients with OAC compared with plasma of patients with BO. Receiver operating characteristic curve analysis revealed that a prediction index calculated by the above-mentioned seven miRNAs could discriminate between patients with OAC and those without OAC with the area under the curve of 0.91, sensitivity of 1 and specificity of 0.75.

Conclusions Levels of the seven circulating miRNAs may represent the tissue miRNA levels and could be promising non-invasive biomarkers to evaluate the carcinogenic process of BO.

  • barrett's oesophagus
  • oesophageal cancer
  • rna expression

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  • Contributors Both authors conceived and designed the experiments. JM performed the experiments and analysed the data. JM wrote the paper, and HS revised the paper.

  • Funding This study was supported by a Grant-in-Aid for Young Scientists (B) (26860527; to JM), a Grant-in-Aid for Scientific Research B (16H05291; to HS) from the Japan Society for the Promotion of Science (JSPS) (, MEXT-Supported Program for the Strategic Research Foundation at Private Universities (S1411003; to HS) (, a grant from Takeda Science Foundation (to JM), the Princess Takamatsu Cancer Research grants (to HS) and the Medical School Faculty and Alumni Grant from Keio University Medical Science Fund (to JM and HS).

  • Disclaimer The funders have no roles in study design, data collection, data analysis, data interpretation or drafting the manuscript. The corresponding author had full access to all the data in the study and had final responsibility for the decision to submit for publication.

  • Competing interests During the last 2 years, HS received scholarship funds for the research from Daiichi-Sankyo Co., EA Pharma Co., Otsuka Pharmaceutical Co. Ltd and Tsumura Co. and received service honoraria from Astellas Pharma Inc., Astra-Zeneca K.K., EA Pharma Co., Otsuka Pharmaceutical Co. Ltd, Daiichi-Sankyo Co., Takeda Pharmaceutical Co. Ltd, Mylan EPD, Co., Tsumura Co. and Zeria Pharmaceutical Co. Ltd.

  • Provenance and peer review Commissioned; externally peer reviewed.

  • Data sharing statement All relevant data are within the paper and its supporting materials.

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