Efficacy of dietary and physical activity intervention in non-alcoholic fatty liver disease: a systematic review
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* Susan Kenneally
* Joanna H Sier
* J Bernadette Moore

## Abstract

**Background** Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide, with prevalence above 30% in many adult populations. Strongly associated with obesity, weight loss through diet and physical activity is the mainstay of its management. Weight loss can be difficult to achieve and maintain however, and uncertainty exists as to which lifestyle changes are most effective.

**Objective** The aim of this work was to systematically evaluate randomised controlled trials assessing diet, exercise or combination interventions aimed at reducing steatosis or markers of NAFLD activity.

**Design** Medline, Scopus and Cochrane databases were searched from 1 January 1980 through to 31 July 2016, for intervention trials assessing the effects of diet, weight loss, exercise or any combination thereof, on NAFLD disease markers in human adults. Risk of publication bias and study quality was assessed using the American Dietetic Association Quality Criteria Checklist.

**Results** From a total of 1710 identified records, 24 articles met the inclusion and exclusion criteria; 6 assessed weight loss using dietary restriction, 10 assessed exercise and 8 were combination interventions. While all of the trials demonstrated significant reduction in steatosis and/or markers of NAFLD activity, combination interventions appear to be the most effective at improving NAFLD. Results suggest that 5–10% weight loss using a modestly hypocaloric diet of 500 kcal less per day than calculated energy requirement, in combination with 30–60 min exercise on 3–5 days per week should be recommended.

**Conclusions** We conclude this amount of weight loss is achievable in the trial setting but is challenging in the clinical environment. High-intensity, multidisciplinary intervention in specialist clinics is likely to be required in order to manage NAFLD by lifestyle modification alone. This systematic review protocol was registered prospectively at PROSPERO as CRD42016032764.

*   FATTY LIVER
*   NONALCOHOLIC STEATOHEPATITIS
*   OBESITY
*   DIET

## Introduction

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of liver pathology, strongly associated with obesity and diabetes. Described as the hepatic component of metabolic syndrome, NAFLD is now the most common cause of chronic liver disease worldwide.1 Prevalence estimates vary depending on population and ethnicity, but current data suggest prevalence may be as high as 45% of the adult population in many countries.2 While the majority of people affected have simple steatosis, which confers a relatively low risk, up to 50% of patients with NAFLD will develop complications including steatohepatitis, fibrosis, cirrhosis and end-stage liver failure or hepatocellular carcinoma.3

Determining the precise incidence and prevalence of NAFLD is problematic as NAFLD is generally an incidental finding in the clinical setting, and the lack of a precise and inexpensive diagnostic tool makes population studies on a large scale difficult.1 A diagnosis of NAFLD can only be made in the absence of significant ethanol intake (<20 g/day in men and 10 g/day in women). In addition, the exclusion of other causes of steatosis, including; hepatitis C, medication, malnutrition, rapid weight loss and inflammatory bowel disease, is also required.4–6 Importantly, evidence suggests it is possible to delay or even reverse disease progression in the earlier stages of NAFLD, and many therapeutic agents and nutritional supplements have been studied to this end, with some showing some limited promise in phase II and III clinical trials.7 However, currently there is no specific pharmaceutical agent for the treatment of NAFLD, and lifestyle change aimed at weight loss remains the mainstay of clinical management guidelines.4–6

Weight loss and increased physical activity are known to be associated with improvements in other components of metabolic syndrome, possibly by reducing the enhanced inflammatory state associated with the condition.8 ,9 While there is general consensus that many types of lifestyle interventions should be effective in reducing steatosis and improving histological markers of disease progression, comparison proves difficult because of the diversity of intervention protocols and NAFLD diagnostic and outcome criteria used in trials.10 Recent meta-analyses examining exercise interventions suggest positive effects on reduction in alanine aminotransferase (ALT) levels and intrahepatic fat, although the clinical significance of reported standard mean differences can be difficult to interpret.11 ,12 These reviews highlight the considerable heterogeneity in duration and types of exercise intervention employed. While they both conclude a benefit to exercise intervention, some low-quality trials were included and questions about inclusion and exclusion criteria, grouping of studies and the role of diet and weight loss remain.

In this context, the aim of this review was to systematically evaluate the existing randomised controlled trials (RCTs) assessing the effects of interventions involving diet, weight loss, exercise or any combination thereof, on biochemical, radiological or histological markers of NAFLD in adults. It was hypothesised that weight loss and exercise interventions would be effective, and that combination interventions would be more effective than weight loss or exercise alone.

## Methods

Methods of the analysis and eligibility criteria were specified prior to the literature search and documented in a protocol registered with Prospero (CRD42016032764). The review was conducted by SK according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines13 including independent execution of search strategy and bias assessment, completed by JS; third-party adjudication was done by JBM. The PubMed, Scopus and the Cochrane databases were searched from 1 January 1980 to 31 July 2016. The search terms were ‘NAFLD and exercise’, ‘NAFLD and weight loss’ and ‘NAFLD and diet/diet, reducing’ for PubMed and Scopus, and ‘NAFLD’ for the Cochrane database. The searches were limited to clinical trials, human studies, English language and journal articles where possible.

RCTs in human adults of any gender/ethnicity with a diagnosis of NAFLD, non-alcoholic steatohepatitis (NASH), fibrosis or NAFLD-based cirrhosis made on validated biochemical, radiological or histological evidence were included; other causes of fatty liver disease were excluded. Papers excluded from this review were: non-human studies, letters/case reports, studies relating to children, articles not reporting primary outcomes of interest or primary data reporting, and trials using non-invasive methods that have not been validated. Included trials had to have measured as primary outcomes either: changes in biochemical, radiological or histological markers of NAFLD in response to a diet, weight loss or exercise (or combination of these) intervention. The validity of eligible RCTs was assessed using the American Dietetic Association (ADA) quality assessment tool.14

Information was recorded from each included trial, specifically: (1) the aim of the intervention; (2) the method of recruiting participants; (3) the inclusion and exclusion criteria for the study; (4) whether or not informed consent was obtained; (5) whether or not ethics approval had been sought and granted; (6) any funding sources; (7) the statistical methods used to analyse the data; (8) methods of randomisation; methods of allocation concealment; blinding of participants and personnel where appropriate; blinding of the outcome assessment; (9) incomplete outcome data reporting; any selective reporting and other sources of bias as detailed in the Cochrane handbook.15

In addition, further information recorded from each trial included: the principal and secondary outcome measures as identified by the authors; the methods of assessing outcome measures; the validity and reliability of the outcome measures; the methods of follow-up for non-respondents; the timing of the outcome assessment and any adverse events. The primary measures of the treatment effects were relative reduction in markers of NAFLD compared with controls. These included degree of steatosis on imaging, levels of biochemical markers of NAFLD, histological assessment of NAFLD activity score (NAS) and change in biochemical indices aimed at assessing degree of NAFLD, for example, the fatty liver index (FLI) or the visceral adiposity index (VAI).

## Results

### Search results

The identification and selection of eligible studies included in this review are illustrated in figure 1. Database searches returned 1979 citations with 269 included in more than one search. Following removal of duplicates, 1710 citations remained. These publications were screened for eligibility by reading the abstracts and those not meeting the eligibility criteria were excluded. Papers excluded were either animal studies, drug/supplementation trials, review papers, trials done in children and trials that were not randomised and/or controlled, trials not assessing NAFLD markers as a primary outcome and non-English articles. This resulted in 43 trials which, based on reading the abstracts, met the inclusion criteria for this review. The papers reporting these trials were then read in full. Following this, a further 19 trials were excluded, for the following reasons: five were not randomised; three had no control or cointervention group; five did not require a diagnosis of NAFLD as an inclusion requirement; one included subjects with hepatitis C so did not meet the exclusion criteria. Two were retrospective studies and three evaluated a primary outcome that was not related to NAFLD. Five trials did not specify NAFLD as an inclusion criterion, but did assess and report change in NAFLD as a main outcome and were included.16–20

![Figure 1](http://bmjopengastro.bmj.com//https://bmjopengastro.bmj.com/content/bmjgast/4/1/e000139/F1.medium.gif)

[Figure 1](http://bmjopengastro.bmj.com//content/4/1/e000139/F1)

Figure 1 
Flow diagram showing identification and selection of relevant studies for inclusion in this systematic review.

### Trial characteristics

Overall, 2322 individuals participated in a total of 24 trials with durations ranging from 2 weeks to 1 year. Of the 24 trials that met the inclusion and exclusion criteria, 620–25 assessed weight loss through dietary modification (table 1), 1016–19 ,26–31 assessed exercise (table 2), and 832–39 investigated the effects of a combination of diet and exercise (table 3).

View this table:
[Table 1](http://bmjopengastro.bmj.com//content/4/1/e000139/T1)

Table 1 
Trials evaluating dietary intervention alone on NAFLD-related outcomes

View this table:
[Table 2](http://bmjopengastro.bmj.com//content/4/1/e000139/T2)

Table 2 
Trials evaluating exercise intervention alone on NAFLD-related outcomes

View this table:
[Table 3](http://bmjopengastro.bmj.com//content/4/1/e000139/T3)

Table 3 
Trials evaluating combination of exercise and dietary intervention on NAFLD outcomes

The protocols for the interventions were diverse. The trials assessing dietary intervention alone were all hypocaloric but reductions in food intake ranged from a mild decrease of 200 kcal/day24 to a very low calorie diet (VLCD) with a total intake of 450 kcal/day.20 Dietary compositions were different for each of the 6 interventions. The majority of exercise interventions were various forms of aerobic exercise such as cycling ergometer16 ,18 or walking at high intensity;19 however three studies considered the effects of differing resistance exercise regimes alone27 ,29 ,30 while the protocol for one involved counselling alone, aimed at increasing physical activity.26 Similarly, there was considerable heterogeneity among the combination interventions.

Inclusion and exclusion criteria also varied; of the 24 trials, 10 specified overweight or obesity as a requirement.16 ,18–20 ,22 ,28 ,34–36 ,39 Diabetes status was sometimes an inclusion criterion16 ,17 but more often a reason for exclusion.18 ,20 ,22 ,24 ,28 ,29 ,32 The majority of trials included participants of both genders (age 18–75), with the percentage participation of each sex varying across the studies, although a minority included exclusively male36 or female35 subjects. Five interventions involving exercise also excluded those who were physically unable to complete the protocol.17 ,29 ,30 ,32 ,34 All but one of the interventions assessing exercise without dietary modification required participants to have been sedentary prior to the intervention.30 The trials took place in a wide range of geographical locations; however, only four studies specified ethnicity as an inclusion/exclusion criterion.16 ,17 ,20 ,33

By our review criteria, outcome measures always included changes in hepatic markers. However, associated or secondary outcomes varied markedly between studies, with some assessing only liver outcomes while others also reported data relating to weight, body mass index, glucose metabolism, cardiovascular fitness and other markers linked with NAFLD. The methods of diagnosing and measuring change in NAFLD were also heterogeneous, the most common being MRI/magnetic resonance spectroscopy (MRS) in eight of the studies;16 ,18 ,19 ,23 ,27 ,28 ,33 ,38 three trials required a biopsy,21 ,34 ,37 five assessed NAFLD using ultrasound,20 ,29 ,31 ,32 ,35 five were based on liver biochemistry,17 ,22 ,26 ,36 ,39 two on ultrasound and liver biochemistry24 ,31 and one on the Asian Working Party Guidelines for the diagnosis of NAFLD.30 ,40 One paper excluded those with simple steatosis and assessed only participants with steatohepatitis.34

### Dietary interventions

Six trials assessing the effects of dietary intervention on NAFLD were identified from: Japan,21 Taiwan,20 Spain,22 USA23 and Iran.24 ,25 Interventions were heterogeneous (table 1) and ranged in duration from 2 weeks to 6 months. Notably five of the six dietary interventions reported liver enzymes and all saw reductions in these after intervention. All measured a weight loss from dietary changes, with most achieving 3.0–4.9%, with the exception of the VLCD trial carried out by Lin *et al*20 where participants only consumed 450 kcal/day or 800 kcal/day. This intervention saw larger weight losses of 9.14% and 8.98%, respectively; with 5 and 10 cases of NAFLD being completely resolved, as assessed by ultrasound. Three of the interventions measured NAFLD severity by ultrasound or nuclear magnetic resonance, and only Lin *et al*20 saw cases resolved; whereas a more moderate calorie restriction diet carried out by Browning *et al*23 saw NAFLD improvement with a 28% intrahepatic triglyceride (IHTG) reduction; and Razavi *et al*25 using a calorie-restricted, dietary approaches to stop hypertension (DASH) diet resulted in 10 participants moving from grade III to II NAFLD and 20 from grade II to I, as assessed by MRS.

### Exercise interventions

Ten RCTs assessing the effects of exercise on NAFLD were identified from: Australia,18 ,19 ,26 UK,27 ,31 USA,28 Italy,16 ,17 Israel29 and Japan.30 Interventions ranged from 4 weeks to 12 months (table 2). Eight trials examined the effects of aerobic exercise, in comparison to a more sedentary lifestyle with only standard care implemented, resulted in improvements in NAFLD17 ,19 ,26–28 ,30 ,31 as measured by reduced levels of hepatic enzymes ALT and aspartate aminotransferase (AST). Six of the 10 interventions also measured and reported percentage change in IHTG content by either proton MRS (H-MRS)19 ,27 ,28 ,31 or MRI.16 ,18 This ranged from 1.76–48%, with the smallest percentage change being associated with the shortest duration intervention of 4 weeks,18 and the largest change being a trial of longer duration at 4 months.31 The interventions carried out by Bacchi *et al*16 and Cuthbertson *et al*31 were both 4 months (16 weeks) in duration with cohorts of similar age and both supervised in exercise achieving 48% and 35% reduction in % IHTG, respectively. However the Bacchi trial defined all three exercise sessions per week as supervised, and the Cuthbertson trial saw participants attend the gym weekly and were monitored through the wellness system which tracked exercise within designated facilities; so the results could be indicative of the beneficial role of direct supervision, in the form of personal trainers.

### Combination interventions

Eight trials that assessed the effects of a combination of dietary and exercise interventions were identified from: Taiwan,32 Germany,33 USA,34 ,37 Mexico,35 Hong Kong38 and Spain39 (table 3). These trials ranged in durations of 10 weeks to 12 months with two exclusively studying women35 or men.36 They all used a combination of hypocaloric diet and regular aerobic exercise, although protocols varied with some also assessing the benefits of resistance exercise. A 9-month study by Kantartzis *et al*33 used dietary counselling and moderate physical activity recommendations, which achieved a 35.2% reduction in liver fat (assessed by MRS), and significant changes in hepatic enzyme levels; resulted in 40% of participants seeing NAFLD resolved. Similarly, in the larger cohort of Wong *et al*,38 154 patients given dietary counselling sessions with aerobic exercise advice over a year, there was a considerable percent change of 54% in IHTG. Other interventions reported the grade of NAFLD rather than % IHTG32 ,34 ,37 but all measured an improvement in their NAFLD grade or NAS. In the six of eight trials that reported changes in body weight over the course of their interventions, five studies showed weight losses of 4.4–8.8%; with Promrat *et al*34 showing the greatest losses in an intensive 48-week intervention.

### Quality assessment

Using the ADA quality assessment criteria (figure 2), 13 of the 24 studies were rated as positive, high-quality trials.16–20 ,24 ,25 ,28 ,29 ,33 ,34 ,37 ,38 In eight of the trials, insufficient information was provided to make an informed decision regarding the adequacy of randomisation21 ,22 ,26 ,27 ,30 ,32 ,35 ,36 and one trial was described as ‘semi-random’.23 One trial rated neutral provided insufficient evidence of group comparability,36 two neutral trials had patients studied in two different centres20 ,31 and one provided insufficient information about the exercise arm of their intervention.35 A number of the trials were rated positive as they met criteria 2, 3, 6 and 7 of the checklist and the majority of the checklist overall, but there were some remaining concerns. In 10 of the trials there was insufficient description of the methods of handling withdrawals from the trial,21–23 ,26 ,27 ,32 ,33 ,36 ,39 nine did not provide information regarding blinding where appropriate, in five there were concerns about the statistical analysis,22–24 ,26 ,33 three provided no discussion of potential limitations23 ,26 ,36 and eight provided insufficient evidence regarding their funding source21–23 ,33 ,35 ,37 ,39 so that it was not possible to reasonably conclude that the results had not been biased as a result of the funding source. In summary, over 50% of trials were of high quality and those rated as neutral did have a number of positive points.

![Figure 2](http://bmjopengastro.bmj.com//https://bmjopengastro.bmj.com/content/bmjgast/4/1/e000139/F2.medium.gif)

[Figure 2](http://bmjopengastro.bmj.com//content/4/1/e000139/F2)

Figure 2 
American Dietetic Association (ADA) Quality Criteria Checklist carried out on the 24 eligible trials. (1) Clear Research Question; (2) Unbiased Selection of Participants; (3) Randomization/Group Comparability; (4) Description of Withdrawals; (5) Blinding; (6) Study Procedures Described; (7) Clearly Defined Outcomes; (8) Appropriate Statistical Analysis; (9) Results Support Conclusion; (10) Funding or Sponsorship Bias Unlikely. To be rated positive, each of criteria 2, 3, 6 and 7 must be met and the majority of 10 criteria overall. Any of criteria 2, 3, 6 and 7 not being met resulted in a neutral rating. The majority of criteria not being met results in a negative rating.

## Discussion

Overall, the evidence from the interventions reviewed suggests that weight loss of 5% in NAFLD or 7–10% in NASH is beneficial, and this should be achieved by a combination of moderate dietary restriction and 30–60 min of moderate-intensity exercise on 3–5 days per week. Moderate-intensity exercise requires moderate effort and noticeable acceleration of the heart rate with some increase in rate of breathing, but the person should still be able to hold a conversation. Moderate-intensity activities include brisk walking, taking stairs, using a cross trainer, gardening or vigorous housework. High-intensity exercise requires a large effort with substantial increase in heart rate and rapid breathing, for example, running or fast cycling; individuals are considerably less able to hold a conversation. The combination of diet and exercise appears to increase the likelihood of achieving the required weight loss and leads to greater improvements in markers of NAFLD activity; current guidelines reflect this.5 ,6 Interventions which improve insulin resistance also improve markers of metabolic syndrome, and dietary restriction and exercise have been shown to improve insulin resistance in trials included in this review.16 ,22 ,24 ,30 ,32 ,33 ,39 However, the relationship may be more complex in that some individuals without insulin resistance or obesity develop NAFLD, and while NAFLD and metabolic syndrome have underlying pathology strongly related to insulin resistance, the relationship may be bi-directional.41

The results of the dietary interventions included in this review suggest that reductions in body weight lead to significant improvements in NAFLD activity. NAS are calculated based on histological grading of patients’ liver steatosis, lobular inflammation and cellular ballooning. The higher the score the greater the NAFLD severity. Four of the trials21–24 achieved comparable weight losses of 4% using a moderately hypocaloric diet. The study involving the VLCD20 achieved a greater loss but these type of diets are a unique intervention in their own right and not intended to be a long-term lifestyle.42 Weight losses on VLCD are typically greater than the 10% recommended for maximal improvement in NAFLD,5 ,6 but the risk of recurring weight gain is significant and weight loss maintenance is challenging in the long term.43 A low carbohydrate diet may improve NAFLD more than other forms of dietary restriction;23 however, evidence is mixed and studies comparing different dietary constituents showed no significant difference between low carbohydrate and low fat diets.22 ,35 ,37

Exercise interventions on the whole resulted in more modest benefits; all of the trials achieved significant improvements in markers of NAFLD activity regardless of the intervention protocol and one16 noted that 23% of subjects experienced resolution of NAFLD. Most of the intervention protocols in this review involved 30–60 min of exercise on 3–5 days per week. The data demonstrate aerobic and resistance exercise are both effective. This is an important finding because resistance exercise is more readily available to those of the population most at risk of NAFLD, that is, those with overweight/obesity. Low cardiorespiratory fitness and numerous medical conditions are often concomitant with this population and can preclude aerobic exercise. Therefore performing resistance exercises, relying on body weight alone, can be effective first steps in improving NAFLD.30 However, while statistically significant, the magnitude of the improvement following exercise interventions appears to be less than in dietary interventions.

Combination interventions appear to be the most effective at improving NAFLD. Greater weight loss was observed in the interventions reviewed, with a range of 4.4–8.8% achieved in these studies.32 ,34 ,35 ,38 ,39 The two longest most intensive intervention trials reported the highest losses in body weight.34 ,38 Al Jiffri *et al*36 did not report body weight but carried out an intervention of 3 months with 100 participants, that resulted in a 15% reduction in BMI; however reasons for this are unclear. At 1200 kcal/day, it was one of the more hypocaloric interventions, with an exercise protocol that was comparable to the other combination interventions. The all-male cohort may be a contributing factor; men lose more weight than women given the same physical activity intervention and are less likely to compensate for energy expended during exercise by increasing energy intake45 ,46 which may result in greater adherence to the dietary component of the intervention. However, the improvement in transaminases that they demonstrated is similar to those in studies achieving lesser weight reductions and 15% BMI losses are unlikely to be achievable in the clinical setting. All of the combination interventions noted a significant improvement in markers of NAFLD; while the interventions were varied in the details, they were broadly similar, measuring improvements in transaminases comparable with those achieved in the weight loss trials. However, transaminase levels do not provide as much information about NAFLD activity as imaging and histology, and are found to be normal in 80% of those with clinically diagnosed NAFLD.47

Evidence that weight loss by energy restriction and exercise leads to an improvement in NAFLD, as discussed here, is consistent in that each of the trials included achieved a significant reduction in one or more markers of NAFLD activity. However, further clarification is needed; trials were varied in size and duration, and the heterogeneity of both these and the intervention designs produced equally diverse results. Such diversity limits the extent to which reliable conclusions can be drawn; nevertheless, evidence-based recommendations regarding weight loss and exercise for the management of NAFLD can still be made while acknowledging that some aspects currently remain incompletely understood. The ability to assess patients with NAFLD in the trial setting is enhanced with more advanced imaging techniques and larger RCTs of longer duration are needed to further investigate the effects of lifestyle on NAFLD.

The mechanisms by which dietary restriction and increased exercise improve NAFLD are not fully understood. Weight loss and exercise are already known to improve other components of metabolic syndrome,48 so the finding that they also improve the hepatic component of this multiorgan disease is not entirely unexpected. Improvements in NAFLD without weight loss may be due to a change of body composition. During interventions involving exercise, fat mass will be lost and muscle mass will be gained, and these opposing changes can result in no overall effect on total body weight. It is possible that it is the fat loss which is important as systemic fat loss is associated with a reduction in IHTG,33 or increased metabolic activity of new muscle mass confers benefits including increased resting metabolic rate.49 An alternative hypothesis is that NAFLD and obesity are inflammatory metabolic states and it is the anti-inflammatory effects of the exercise that lead to the improvements seen.9 ,50

In conclusion, this review has found that interventions involving a combination of dietary restriction and exercise, associated with weight loss, are effective in reducing NAFLD activity, in many cases leading to resolution of the condition. Patients with NAFLD should be advised to aim to lose 5–10% of their total body weight by eating a modestly hypocaloric (−500 kcal per day) diet and engaging in regular exercise for 30–60 min on 3–5 days per week. Dietary composition is probably less important than total energy intake, although a low-carbohydrate diet may be slightly more effective than the alternatives if it is acceptable to the individual. Aerobic and resistance exercise both appear to be equally effective, but less so alone without dietary intervention. Achieving this amount of weight loss is challenging in the existing obesogenic environment and clinical intervention is needed, either in a tier 3 multidisciplinary weight management clinic or via bariatric surgery, which is beyond the scope of this review. Tier 3 clinics are currently underdeveloped to meet existing need and an increase in this type of service is desirable, to support patients and reduce NAFLD progression.

## Footnotes

*   SK and JHS are joint first authors.

*   This protocol was registered at PROSPERO database ([https://www.crd.york.ac.uk/PROSPERO/](https://www.crd.york.ac.uk/PROSPERO/)) as CRD42016032764 ([https://www.crd.york.ac.uk/PROSPERO/display_record.asp?ID=CRD42016032764](https://www.crd.york.ac.uk/PROSPERO/display_record.asp?ID=CRD42016032764)).

*   Contributors SK and JBM designed this research. SK and JHS conducted this review, analysed the data and contributed equally to drafting the paper. JBM had primary responsibility for final content.

*   Competing interests None declared.

*   Provenance and peer review Not commissioned; externally peer reviewed.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: [http://creativecommons.org/licenses/by-nc/4.0/](http://creativecommons.org/licenses/by-nc/4.0/)

## References

1.  Moore JB. Non-alcoholic fatty liver disease: the hepatic consequence of obesity and the metabolic syndrome. Proc Nutr Soc 2010;69:211–20. [doi:10.1017/S0029665110000030](http://dx.doi.org/10.1017/S0029665110000030)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1017/S0029665110000030&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=20158939&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

2.  Farrell GC, Wong VW, Chitturi S. NAFLD in Asia–as common and important as in the West. Nat Rev Gastroenterol Hepatol 2013;10:307–18. [doi:10.1038/nrgastro.2013.34](http://dx.doi.org/10.1038/nrgastro.2013.34)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1038/nrgastro.2013.34&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=23458891&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

3.  Ekstedt M, Hagström H, Nasr P, et al. Fibrosis stage is the strongest predictor for disease-specific mortality in NAFLD after up to 33 years of follow-up. Hepatology 2015;61:1547–54. [doi:10.1002/hep.27368](http://dx.doi.org/10.1002/hep.27368)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/hep.27368&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=25125077&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

4.  Chalasani N, Younossi Z, Lavine JE, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice guideline by the American Gastroenterological Association, American Association for the Study of Liver Diseases, and American College of Gastroenterology. Gastroenterology 2012;142:1592–609. [doi:10.1053/j.gastro.2012.04.001](http://dx.doi.org/10.1053/j.gastro.2012.04.001)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1053/j.gastro.2012.04.001&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=22656328&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000304778700037&link_type=ISI) 

5.  EASL. Clinical Practice Guidelines for the management of non-alcoholic fatty liver disease. J Hepatol 2013;64:1388–402.
    
    

6.  National Guideline C. National Institute for Health and Care Excellence: Guidance. Non-Alcoholic Fatty Liver Disease: Assessment and Management. UK: National Institute for Health and Care Excellence, 2016.
    
    

7.  Wong VW-S, Chitturi S, Wong GL-H, et al. Pathogenesis and novel treatment options for non-alcoholic steatohepatitis. Lancet Gastroenterol Hepatol 2016;1:56–67. [doi:10.1016/S2468-1253(16)30011-5](http://dx.doi.org/10.1016/S2468-1253(16)30011-5)
    
    

8.  Musso G, Cassader M, Rosina F, et al. Impact of current treatments on liver disease, glucose metabolism and cardiovascular risk in non-alcoholic fatty liver disease (NAFLD): a systematic review and meta-analysis of randomised trials. Diabetologia 2012;55:885–904. [doi:10.1007/s00125-011-2446-4](http://dx.doi.org/10.1007/s00125-011-2446-4)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1007/s00125-011-2446-4&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=22278337&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000301182000006&link_type=ISI) 

9.  Ringseis R, Eder K, Mooren FC, et al. Metabolic signals and innate immune activation in obesity and exercise. Exerc Immunol Rev 2015;21:58–68.
    
    

10. Hannah WN, Jr., Harrison SA. Effect of weight loss, diet, exercise, and bariatric surgery on nonalcoholic fatty liver disease. Clin Liver Dis 2016;20:339–50. [doi:10.1016/j.cld.2015.10.008](http://dx.doi.org/10.1016/j.cld.2015.10.008)
    
    

11. Orci LA, Gariani K, Oldani G, et al. Exercise-based interventions for nonalcoholic fatty liver disease: a meta-analysis and meta-regression. Clin Gastroenterol Hepatol 2016;14:1398–411. [doi:10.1016/j.cgh.2016.04.036](http://dx.doi.org/10.1016/j.cgh.2016.04.036)
    
    

12. Katsagoni CN, Georgoulis M, Papatheodoridis GV, et al. Effects of lifestyle interventions on clinical characteristics of patients with non-alcoholic fatty liver disease: a meta-analysis. Metabolism 2017;68:119–32. [doi:10.1016/j.metabol.2016.12.006](http://dx.doi.org/10.1016/j.metabol.2016.12.006)
    
    

13. Moher D, Liberati A, Tetzlaff J, et al. Reprint–preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. Phys Ther 2009;89:873–80.
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6OToicHRqb3VybmFsIjtzOjU6InJlc2lkIjtzOjg6Ijg5LzkvODczIjtzOjQ6ImF0b20iO3M6MjU6Ii9ibWpnYXN0LzQvMS9lMDAwMTM5LmF0b20iO31zOjg6ImZyYWdtZW50IjtzOjA6IiI7fQ==) 

14. Association AD. ADA Evidence Analysis Manual. Ed IV. 2003.
    
    

15. Higgins JPT, Green S. Cochrane handbook for systematic reviews of interventions version 5.1.0. The Cochrane Collaboration, 2011.
    
    

16. Bacchi E, Negri C, Targher G, et al. Both resistance training and aerobic training reduce hepatic fat content in type 2 diabetic subjects with nonalcoholic fatty liver disease (the RAED2 Randomized Trial). Hepatology 2013;58:1287–95. [doi:10.1002/hep.26393](http://dx.doi.org/10.1002/hep.26393)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/hep.26393&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=23504926&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

17. Balducci S, Cardelli P, Pugliese L, et al. Volume-dependent effect of supervised exercise training on fatty liver and visceral adiposity index in subjects with type 2 diabetes The Italian Diabetes Exercise Study (IDES). Diabetes Res Clin Pract 2015;109:355–63. [doi:10.1016/j.diabres.2015.05.033](http://dx.doi.org/10.1016/j.diabres.2015.05.033)
    
    

18. Johnson NA, Sachinwalla T, Walton DW, et al. Aerobic exercise training reduces hepatic and visceral lipids in obese individuals without weight loss. Hepatology 2009;50:1105–12. [doi:10.1002/hep.23129](http://dx.doi.org/10.1002/hep.23129)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/hep.23129&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=19637289&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000270455800017&link_type=ISI) 

19. Keating SE, Hackett DA, Parker HM, et al. Effect of aerobic exercise training dose on liver fat and visceral adiposity. J Hepatol 2015;63:174–82. [doi:10.1016/j.jhep.2015.02.022](http://dx.doi.org/10.1016/j.jhep.2015.02.022)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1016/j.jhep.2015.02.022&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=25863524&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

20. Lin WY, Wu CH, Chu NF, et al. Efficacy and safety of very-low-calorie diet in Taiwanese: a multicenter randomized, controlled trial. Nutrition 2009;25:1129–36. [doi:10.1016/j.nut.2009.02.008](http://dx.doi.org/10.1016/j.nut.2009.02.008)
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=19592223&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

21. Yamamoto M, Iwasa M, Iwata K, et al. Restriction of dietary calories, fat and iron improves non-alcoholic fatty liver disease. J Gastroenterol Hepatol 2007;22:498–503. [doi:10.1111/j.1440-1746.2006.04548.x](http://dx.doi.org/10.1111/j.1440-1746.2006.04548.x)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1111/j.1440-1746.2006.04548.x&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=17376040&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000246263600009&link_type=ISI) 

22. de Luis DA, Aller R, Izaola O, et al. Effect of two different hypocaloric diets in transaminases and insulin resistance in nonalcoholic fatty liver disease and obese patients. Nutr Hosp 2010;25:730–5.
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=21336428&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

23. Browning JD, Baker JA, Rogers T, et al. Short-term weight loss and hepatic triglyceride reduction: evidence of a metabolic advantage with dietary carbohydrate restriction. Am J Clin Nutr 2011;93: 1048–52. [doi:10.3945/ajcn.110.007674](http://dx.doi.org/10.3945/ajcn.110.007674)
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NDoiYWpjbiI7czo1OiJyZXNpZCI7czo5OiI5My81LzEwNDgiO3M6NDoiYXRvbSI7czoyNToiL2Jtamdhc3QvNC8xL2UwMDAxMzkuYXRvbSI7fXM6ODoiZnJhZ21lbnQiO3M6MDoiIjt9) 

24. Kani AH, Alavian SM, Esmaillzadeh A, et al. Effects of a novel therapeutic diet on liver enzymes and coagulating factors in patients with non-alcoholic fatty liver disease: a parallel randomized trial. Nutrition 2014;30:814–21. [doi:10.1016/j.nut.2013.11.008](http://dx.doi.org/10.1016/j.nut.2013.11.008)
    
    

25. Razavi Zade M, Telkabadi MH, Bahmani F, et al. The effects of DASH diet on weight loss and metabolic status in adults with non-alcoholic fatty liver disease: a randomized clinical trial. Liver Int 2016;36:563–71. [doi:10.1111/liv.12990](http://dx.doi.org/10.1111/liv.12990)
    
    

26. St George A, Bauman A, Johnston A, et al. Effect of a lifestyle intervention in patients with abnormal liver enzymes and metabolic risk factors. J Gastroenterol Hepatol 2009;24:399–407. [doi:10.1111/j.1440-1746.2008.05694.x](http://dx.doi.org/10.1111/j.1440-1746.2008.05694.x)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1111/j.1440-1746.2008.05694.x&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=19067776&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

27. Hallsworth K, Fattakhova G, Hollingsworth KG, et al. Resistance exercise reduces liver fat and its mediators in non-alcoholic fatty liver disease independent of weight loss. Gut 2011;60:1278–83. [doi:10.1136/gut.2011.242073](http://dx.doi.org/10.1136/gut.2011.242073)
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NjoiZ3V0am5sIjtzOjU6InJlc2lkIjtzOjk6IjYwLzkvMTI3OCI7czo0OiJhdG9tIjtzOjI1OiIvYm1qZ2FzdC80LzEvZTAwMDEzOS5hdG9tIjt9czo4OiJmcmFnbWVudCI7czowOiIiO30=) 

28. Sullivan S, Kirk EP, Mittendorfer B, et al. Randomized trial of exercise effect on intrahepatic triglyceride content and lipid kinetics in nonalcoholic fatty liver disease. Hepatology 2012;55:1738–45. [doi:10.1002/hep.25548](http://dx.doi.org/10.1002/hep.25548)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/hep.25548&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=22213436&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000304530200012&link_type=ISI) 

29. Zelber-Sagi S, Buch A, Yeshua H, et al. Effect of resistance training on non-alcoholic fatty-liver disease a randomized-clinical trial. World J Gastroenterol 2014;20:4382–92. [doi:10.3748/wjg.v20.i15.4382](http://dx.doi.org/10.3748/wjg.v20.i15.4382)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.3748/wjg.v20.i15.4382&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=24764677&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

30. Takahashi A, Abe K, Usami K, et al. Simple resistance exercise helps patients with non-alcoholic fatty liver disease. Int J Sports Med 2015;36:848–52. [doi:10.1055/s-0035-1549853](http://dx.doi.org/10.1055/s-0035-1549853)
    
    

31. Cuthbertson DJ, Shojaee-Moradie F, Sprung VS, et al. Dissociation between exercise-induced reduction in liver fat and changes in hepatic and peripheral glucose homoeostasis in obese patients with non-alcoholic fatty liver disease. Clin Sci (Lond) 2016;130:93–104. [doi:10.1042/CS20150447](http://dx.doi.org/10.1042/CS20150447)
    
    

32. Chen SM, Liu CY, Li SR, et al. Effects of therapeutic lifestyle program on ultrasound-diagnosed nonalcoholic fatty liver disease. J Chin Med Assoc 2008;71:551–8. [doi:10.1016/S1726-4901(08)70168-0](http://dx.doi.org/10.1016/S1726-4901(08)70168-0)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1016/S1726-4901(08)70168-0&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=19015052&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

33. Kantartzis K, Thamer C, Peter A, et al. High cardiorespiratory fitness is an independent predictor of the reduction in liver fat during a lifestyle intervention in non-alcoholic fatty liver disease. Gut 2009;58:1281–8. [doi:10.1136/gut.2008.151977](http://dx.doi.org/10.1136/gut.2008.151977)
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NjoiZ3V0am5sIjtzOjU6InJlc2lkIjtzOjk6IjU4LzkvMTI4MSI7czo0OiJhdG9tIjtzOjI1OiIvYm1qZ2FzdC80LzEvZTAwMDEzOS5hdG9tIjt9czo4OiJmcmFnbWVudCI7czowOiIiO30=) 

34. Promrat K, Kleiner DE, Niemeier HM, et al. Randomized controlled trial testing the effects of weight loss on nonalcoholic steatohepatitis. Hepatology 2010;51:121–9. [doi:10.1002/hep.23276](http://dx.doi.org/10.1002/hep.23276)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/hep.23276&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=19827166&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000273438400017&link_type=ISI) 

35. Rodriguez-Hernandez H, Cervantes-Huerta M, Rodriguez-Moran M, et al. Decrease of aminotransferase levels in obese women is related to body weight reduction, irrespective of type of diet. Ann Hepatol 2011;10:486–92.
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=21911890&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

36. Al-Jiffri O, Al-Sharif FM, Abd El-Kader SM, et al. Weight reduction improves markers of hepatic function and insulin resistance in type-2 diabetic patients with non-alcoholic fatty liver. Afr Health Sci 2013;13:667–72. [doi:10.4314/ahs.v13i3.21](http://dx.doi.org/10.4314/ahs.v13i3.21)
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=24250305&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

37. Eckard C, Cole R, Lockwood J, et al. Prospective histopathologic evaluation of lifestyle modification in nonalcoholic fatty liver disease: a randomized trial. Therap Adv Gastroenterol 2013;6:249–59. [doi:10.1177/1756283X13484078](http://dx.doi.org/10.1177/1756283X13484078)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1177/1756283X13484078&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=23814606&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

38. Wong VW, Chan RS, Wong GL, et al. Community-based lifestyle modification programme for non-alcoholic fatty liver disease: a randomized controlled trial. J Hepatol 2013;59:536–42. [doi:10.1016/j.jhep.2013.04.013](http://dx.doi.org/10.1016/j.jhep.2013.04.013)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1016/j.jhep.2013.04.013&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=23623998&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000323602000023&link_type=ISI) 

39. Aller R, de Luis DA, Izaola O, et al. Effect of a high monounsaturated vs high polyunsaturated fat hypocaloric diets in nonalcoholic fatty liver disease. Eur Rev Med Pharmacol Sci 2014;18:1041–7.
    
    

40. Chitturi S, Farrell GC, Hashimoto E, et al. Non-alcoholic fatty liver disease in the Asia-Pacific region: definitions and overview of proposed guidelines. J Gastroenterol Hepatol 2007;22:778–87. [doi:10.1111/j.1440-1746.2007.05001.x](http://dx.doi.org/10.1111/j.1440-1746.2007.05001.x)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1111/j.1440-1746.2007.05001.x&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=17565630&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000247205600005&link_type=ISI) 

41. Wainwright P, Byrne CD. Bidirectional relationships and disconnects between NAFLD and features of the metabolic syndrome. Int J Mol Sci 2016;17:367. [doi:10.3390/ijms17030367](http://dx.doi.org/10.3390/ijms17030367)
    
    

42. Tsai AG, Wadden TA. The evolution of very-low-calorie diets: an update and meta-analysis. Obesity (Silver Spring) 2006;14: 1283–93. [doi:10.1038/oby.2006.146](http://dx.doi.org/10.1038/oby.2006.146)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1038/oby.2006.146&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=16988070&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 

43. Johansson K, Neovius M, Hemmingsson E. Effects of anti-obesity drugs, diet, and exercise on weight-loss maintenance after a very-low-calorie diet or low-calorie diet: a systematic review and meta-analysis of randomized controlled trials. Am J Clin Nutr 2014;99:14–23. [doi:10.3945/ajcn.113.070052](http://dx.doi.org/10.3945/ajcn.113.070052)
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NDoiYWpjbiI7czo1OiJyZXNpZCI7czo3OiI5OS8xLzE0IjtzOjQ6ImF0b20iO3M6MjU6Ii9ibWpnYXN0LzQvMS9lMDAwMTM5LmF0b20iO31zOjg6ImZyYWdtZW50IjtzOjA6IiI7fQ==) 

44. Keating SE, Hackett DA, George J, et al. Exercise and non-alcoholic fatty liver disease: a systematic review and meta-analysis. J Hepatol 2012;57:157–66. [doi:10.1016/j.jhep.2012.02.023](http://dx.doi.org/10.1016/j.jhep.2012.02.023)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1016/j.jhep.2012.02.023&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=22414768&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000305811300023&link_type=ISI) 

45. Williams RL, Wood LG, Collins CE, et al. Effectiveness of weight loss interventions--is there a difference between men and women: a systematic review. Obes Rev 2015;16:171–86. [doi:10.1111/obr.12241](http://dx.doi.org/10.1111/obr.12241)
    
    

46. Stubbs RJ, Sepp A, Hughes DA, et al. The effect of graded levels of exercise on energy intake and balance in free-living men, consuming their normal diet. Eur J Clin Nutr 2002;56:129–40. [doi:10.1038/sj.ejcn.1601295](http://dx.doi.org/10.1038/sj.ejcn.1601295)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1038/sj.ejcn.1601295&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=11857046&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000173810400006&link_type=ISI) 

47. Szczepaniak LS, Nurenberg P, Leonard D, et al. Magnetic resonance spectroscopy to measure hepatic triglyceride content: prevalence of hepatic steatosis in the general population. Am J Physiol Endocrinol Metab 2005;288:E462–8. [doi:10.1152/ajpendo.00064.2004](http://dx.doi.org/10.1152/ajpendo.00064.2004)
    
    [Abstract/FREE Full Text](http://bmjopengastro.bmj.com//lookup/ijlink/YTozOntzOjQ6InBhdGgiO3M6MTQ6Ii9sb29rdXAvaWpsaW5rIjtzOjU6InF1ZXJ5IjthOjQ6e3M6ODoibGlua1R5cGUiO3M6NDoiQUJTVCI7czoxMToiam91cm5hbENvZGUiO3M6NzoiYWpwZW5kbyI7czo1OiJyZXNpZCI7czoxMDoiMjg4LzIvRTQ2MiI7czo0OiJhdG9tIjtzOjI1OiIvYm1qZ2FzdC80LzEvZTAwMDEzOS5hdG9tIjt9czo4OiJmcmFnbWVudCI7czowOiIiO30=) 

48. Beavers KM, Nicklas BJ. Effects of lifestyle interventions on inflammatory markers in the metabolic syndrome. Front Biosci 2011;3:168–77. [doi:10.2741/s142](http://dx.doi.org/10.2741/s142)
    
    

49. Speakman JR, Selman C. Physical activity and resting metabolic rate. Proc Nutr Soc 2003;62:621–34. [doi:10.1079/PNS2003282](http://dx.doi.org/10.1079/PNS2003282)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1079/PNS2003282&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=14692598&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom) 
    
    [Web of Science](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=000185487500008&link_type=ISI) 

50. Wensveen FM, Valentić S, Šestan M, et al. The “Big Bang” in obese fat: Events initiating obesity-induced adipose tissue inflammation. Eur J Immunol 2015;45:2446–56. [doi:10.1002/eji.201545502](http://dx.doi.org/10.1002/eji.201545502)
    
    [CrossRef](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=10.1002/eji.201545502&link_type=DOI) 
    
    [PubMed](http://bmjopengastro.bmj.com//lookup/external-ref?access_num=26220361&link_type=MED&atom=%2Fbmjgast%2F4%2F1%2Fe000139.atom)