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Comparative gastrointestinal and plasma cholesterol responses of rats fed on cholesterol-free diets supplemented with guar gum and sodium alginate

Published online by Cambridge University Press:  09 March 2007

C. J. Seal  and
J. C. Mathers
C. J. Seal*
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
J. C. Mathers
Affiliation:
Human Nutrition Research Centre, Department of Biological and Nutritional Sciences, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
*
*Corresponding author: Dr C. J. Seal, fax +44 191 222 8684, email chris.seal@ncl.ac.uk
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Abstract

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The present study investigated the digestion and cholesterol-lowering effects of the water-soluble NSP guar gum (GG) and sodium alginate (SA) in laboratory animals. Groups of five male Wistar strain rats were fed semi-purified cholesterol-free diets containing 0, 50 or 100 g NSP source/kg for 21 d which comprised a 14-d adaptation period followed by a 7-d balance period. Weight gain over the balance period and food conversion ratio decreased linearly with increasing NSP intake (P=0.006 and P=0.07 respectively). DM digestibility decreased with increasing NSP intake (P=0.001) and this effect was greater for SA-containing diets compared with GG-containing diets (P=0.001). At the lower inclusion rate, 0.9–1.0 of the additional NSP was digested, but this value fell to 0.8 for both NSP sources at the 100 g/kg inclusion rate, implying that the capacity for near complete digestion of the test NSP had been exceeded. Intestinal tissue mass was increased in response to inclusion of both NSP sources. Caecal digesta pH decreased linearly with additional GG, but increased slightly with consumption of SA. Total caecal short-chain fatty acid concentrations (μmol/g caecal contents) increased markedly with 50 g GG/kg but did not increase further with 100 g GG/kg, and were slightly lower than control values in rats consuming SA. Plasma cholesterol concentration fell linearly (P=0.03) with increasing NSP in the diet and the effect was similar for both GG and SA. Total output of faecal bile acids rose in rats fed 50 g GG/kg and 50 g SA/kg (59 μmol/7 d v. 24 μmol/7 d for control rats) with no further increase at the higher inclusion rate. These results show that SA has a strong hypocholesterolaemic effect in rats which is similar to that of GG, and that this effect is most likely to be mediated through an interruption in the entero-hepatic circulation of bile acids and not through increased hepatic supply of propionate from fermentation of the NSP in the large bowel.

Keywords

Sodium alginateCholesterol metabolismBile acidsNSP digestionCaecal fermentation
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 3 , March 2001 , pp. 317 - 324
Copyright
Copyright © The Nutrition Society 2001

References

Anderson, JW (1985) Physiological and metabolic effects of dietary fibre. Federation Proceedings 44, 2902–2906.Google Scholar
Beaulieu, KE & McBurney, MI (1992) Changes in pig serum lipids, nutrient digestibility and sterol excretion during cecal infusion of propionate. Journal of Nutrition 122, 241–245.CrossRefGoogle ScholarPubMed
BeMiller, JN & Whistler, RL (1996) Carbohydrates Food Chemistry, 3rd ed., pp. 157–223[OM, Fennema, editor]. New York: Marcel Dekker.Google Scholar
Bosaeus, I, Carlsson, N-G, Sandberg, A-S & Andersson, H (1986) Effect of wheat bran and pectin on bile acid and cholesterol excretion in ileostomy patients. Human Nutrition Clinical Nutrition 40, 429–440.Google ScholarPubMed
Brown, L, Willett, WW & Sacks, FM (1999) Cholesterol-lowering effects of dietary fiber: a meta-analysis American Journal of Clinical Nutrition 69, 30–42.CrossRefGoogle ScholarPubMed
Buhman, KK, Furumoto, EJ, Donkin, SS & Story, JA (1998) Dietary psyllium increases fecal bile acid excretion, total steroid excretion and bile acid biosynthesis in rats. Journal of Nutrition 128, 1199–1203.CrossRefGoogle ScholarPubMed
Carr, TP, Gallaher, DD, Yang, C-H & Hassel, CA (1996) Increased intestinal contents viscosity reduces cholesterol absorption efficiency in hamsters fed hydroxypropyl methylcellulose. Journal of Nutrition 126, 1463–1469.Google Scholar
Chen, WL, Anderson, JW & Jennings, D (1984) Propionate may mediate the hypocholesterolemic effect of certain soluble plant fibres in cholesterol fed rats. Proceedings of the Society for Experimental Biology and Medicine 175, 215–218.CrossRefGoogle ScholarPubMed
Chiang, JYL, Miller, WF & Lin, G-M (1990) Regulation of cholesterol 7α-hydroxylase and the immunological evidence for the induction of cholesterol 7α-hydroxylase by cholestyramine and circadian rhythm. Journal of Biological Chemistry 265, 3889–3897.CrossRefGoogle Scholar
Demigné, C, Morand, C, Levrat, M-A, Besson, C, Moundras, C & Rémésy, C (1995) Effect of propionate on fatty acid and cholesterol synthesis and on acetate metabolism in isolated rat hepatocytes. British Journal of Nutrition 74, 209–219.CrossRefGoogle ScholarPubMed
Englyst, HN & Cummings, JH (1984) Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as the alditol acetates. Analyst 109, 937–942.CrossRefGoogle Scholar
Fairweather #Tait, SJ, Gee, JM & Johnson, IT (1983) The influence of cooked kidney beans (Phaseolus vulgaris) on intestinal cell turnover and faecal nitrogen excretion in the rat. British Journal of Nutrition 49, 303–312.CrossRefGoogle ScholarPubMed
Fernandez, ML (1995) Distinct mechanisms of plasma LDL lowering by dietary fibre in the guinea pig: specific effects of pectin, guar gum and psyllium. Journal of Lipid Research. 36, 2394–2404.CrossRefGoogle ScholarPubMed
Frape, DL & Jones, AM (1995) Chronic and postprandial responses of plasma insulin, glucose and lipids in volunteers given dietary fibre supplements. British Journal of Nutrition 73, 733–751.Google Scholar
Gallaher, DD, Hassel, CA, Lee, K-J & Gallaher, CM (1993) Viscosity and fermentability as attributes of dietary fiber responsible for the hypocholesterolemic effect in hamsters. Journal of Nutrition 123, 244–252.Google ScholarPubMed
Gallaher, DD, Locket, PL & Gallaher, CM (1992) Bile acid metabolism in rats fed two levels of corn oil and brans of oat, rye and barley and sugar beet fiber. Journal of Nutrition 122, 473–481.CrossRefGoogle ScholarPubMed
Goodlad, JS & Mathers, JC (1990) Large bowel fermentation in rats given diets containing raw peas (Pisum sativum). British Journal of Nutrition 64, 569–587.Google Scholar
Goodlad, JS & Mathers, JC (1992) Digestion of complex carbohydrates and large bowel fermentation in rats fed on raw & cooked peas (Pisum sativum). British Journal of Nutrition 67, 475–488.CrossRefGoogle ScholarPubMed
Hara, H, Haga, S, Aoyama, Y & Kiriyama, S (1998) Short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine. Journal of Nutrition 129, 942–948.CrossRefGoogle Scholar
Illman, RJ, Topping, DL, McIntosh, GH, Trimble, RP, Storer, GB, Taylor, MN & Cheng, BQ (1988) Hypocholesterolemic effects of dietary propionate; studies in whole animals and perfused rat liver. Annals of Nutrition and Metabolism 32, 97–107.CrossRefGoogle Scholar
Jenkins, DJA, Reynolds, D, Slavin, B, Leeds, AR, Jenkins, AL & Jepson, EH (1980) Dietary fiber and blood lipids: treatment of hypocholesterolaemia with guar crispbread. American Journal of Clinical Nutrition 33, 575–581.CrossRefGoogle Scholar
Key, FB & Mathers, JC (1993) Gastrointestinal responses of rats fed on white and wholemeal breads: complex carbohydrate digestibility and the influence of dietary fat content. British Journal of Nutrition 69, 481–495.CrossRefGoogle ScholarPubMed
Key, FB & Mathers, JC (1993) Complex carbohydrate digestion and large bowel fermentation in rats given wholemeal bread and cooked haricot beans (Phaseolus vulgaris). fed in mixed diets British Journal of Nutrition 69, 497–509.CrossRefGoogle ScholarPubMed
Key, FB & Mathers, JC (1995) Digestive adaptations of rats given white bread and cooked haricot beans (Phaseolus vulgaris): large bowel fermentation and digestion of complex carbohydrates. British Journal of Nutrition 74, 393–406.Google Scholar
Levrat, M-A, Favier, M-L, Moundras, C, Rémésy, C, Demigné, C & Morand, C (1994) Role of dietary propionic acid and bile acid excretion in the hypocholesterolemic effects of oligosaccharides in rats. Journal of Nutrition 124, 531–538.CrossRefGoogle ScholarPubMed
Lin, Y, Vonk, RJ, Sloof, MJH, Kuipers, F & Smit, MJ (1995) Differences in propionate-induced inhibition of cholesterol and triacylglycerol synthesis between human and rat hepatocytes in primary culture. British Journal of Nutrition 74, 197–207.Google Scholar
Martin, G (1986) Evaluation toxilogique et nutritionelle des alginates. Définition, structure, fabrication propiétés et applications (Toxicological and nutritional evaluation of alginates, Definition, structure, manufacturing properties and applications) Science Aliments 6, 473–486.Google Scholar
Mason, VC & Palmer, R (1973) The influence of bacterial activity in the alimentary canal of rats on faecal nitrogen excretion. Acta Agriculturae Scandinavica 23, 141–150.CrossRefGoogle Scholar
Mathers, JC (1991) Digestion on non-starch polysaccharides by non-ruminant omnivores. Proceedings of the Nutrition Society 50, 161–172.CrossRefGoogle ScholarPubMed
Mathers, JC, Fernandez, F, Hill, MJ, McCarthy, PT, Shearer, MJ & Oxley, A (1990) Dietary modification of potential vitamin K supply from enteric bacteria menaquinones in rats. British Journal of Nutrition 63, 639–652.CrossRefGoogle ScholarPubMed
Michel, C, Lahaye, M, Bonnet, C, Mabeau, S & Barry, JL (1996) In vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds. British Journal of Nutrition 75, 263–280.CrossRefGoogle ScholarPubMed
Michel, C & Macfarlane, GT (1996) Digestive fates of soluble polysaccharides from marine macroalgae: involvement of the colonic microflora and physiological consequences for the host. Journal of Applied Bacteriology 80, 349–369.Google Scholar
Morgan, LM, Tredger, JA, Shavila, Y, Travis, JS & Wright, J (1993) The effect of non-starch polysaccharide supplementation on circulating bile acids, hormone and metabolite levels following a fat meal in human subjects. British Journal of Nutrition 70, 491–501.CrossRefGoogle ScholarPubMed
Moundras, C, Behr, SR, Rémésy, C & Demigné, C (1997) Fecal losses of sterols and bile acids induced by feeding rats guar gum are due to greater pool size and liver bile acid secretion. Journal of Nutrition 127, 1068–1076.CrossRefGoogle ScholarPubMed
Nyman, M, Asp, N-G, Cummings, J & Wiggins, H (1986) Fermentation of dietary fibre in the intestinal tract: comparison between man and rat. British Journal of Nutrition 55, 487–496.Google Scholar
Overton, PD, Furlonger, N, Beety, JM, Chakraborty, J, Tredger, JA & Morgan, IM (1994) The effects of dietary sugar-beet fibre and guar gum on lipid metabolism in Wistar rats. British Journal of Nutrition 72, 385–395.Google Scholar
Rodwell, VW, Nordstrom, JL & Mitschelen, JJ (1976) Regulation of HMG-CoA reductase. Advances in Lipid Research 14, 1–74.CrossRefGoogle ScholarPubMed
Roper, CS, Bal, W & Mathers, JC (1996) Comparison of two algal polysaccharides with guar gum and cellulose on bowel habit in healthy human volunteers. Proceedings of the Nutrition Society 55, 238A.Google Scholar
Sandberg, AS, Andersson, H, Bosaeus, I, Carlsson, NG, Hasselblad, K & Harrod, M (1994) Alginate, small bowel sterol excretion, and absorption of nutrients in ileostomy subjects. American Journal of Clinical Nutrition 60, 751–756.CrossRefGoogle ScholarPubMed
Seal, CJ & Mathers, JC (1996) Comparative gastrointestinal responses to guar gum and a seaweed polysaccharide (sodium alginate) in rats. Proceedings of the Nutrition Society 55, 55A.Google Scholar
Setchell, KDR, Lawson, AM, Tamida, N & Sjövall, J (1983) General methods for the analysis of metabolic profiles of bile acids and related compounds in faeces. Journal of Lipid Research 24, 1085–1100.CrossRefGoogle Scholar
Skurpakkar, KS, Sundaravalli, OE & Rao, MN (1979) In vitro and in vivo digestibility of legume carbohydrates. Nutrition Reports International 19, 111–117.Google Scholar
Story, JA, Furumoto, EJ, Buhman, KK (1997) Dietary fiber and bile acid metabolism – an update. In Dietary Fibre in Health and Disease, 259–266. [D, Krichevsky and C, Bonfield, editors]. New York: Plenum Press.Google Scholar
Todesco, T, Rao, AV, Bosello, O & Jenkins, DJA (1991) Propionate lowers blood glucose and alters lipid metabolism in health subjects. American Journal of Clinical Nutrition 54, 860–869.CrossRefGoogle Scholar
Trautwein, EA, Kunath-Rau, A & Erbersdober, HF (1998) Effect of different varieties of pectin and guar gum on plasma, hepatic and biliary lipids and cholesterol gallstone formation in hamsters fed on high-cholesterol diets. British Journal of Nutrition 79, 463–471.CrossRefGoogle ScholarPubMed
Trautwein, EA, Kunath-Rau, A & Erbersdobler, HF (1999) Increased fecal bile acid excretion and changes in the circulating bile acid pool are involved in the hypocholesterolemic and gallstone-preventive actions of psyllium in hamsters. Journal of Nutrition 129, 896–902.CrossRefGoogle ScholarPubMed
Tredger, JA, Morgan, LM, Travis, J & Marks, V (1991) The effects of guar gum, sugar beet fibre and wheat bran supplementation on serum lipoprotein levels in normocholesterolaemic volunteers. Journal of Human Nutrition and Dietetics 4, 375–384.Google Scholar
Truswell, AS (1995) Dietary fibre and plasma lipids. European Journal of Clinical Nutrition 49, S105-S109.Google ScholarPubMed
Venter, CS, Vorster, HH & Cummings, JH (1990) Effects of dietary propionate on carbohydrate and lipid metabolism in healthy volunteers. American Journal of Gastroenterology 85, 549–553.Google ScholarPubMed
Wu, J & Peng, SS (1997) Comparison of hypolipidemic effect of refined konjac meal with several common dietary fibers and their mechanisms of action. Biomedical and Environmental Sciences 10, 27–37.Google Scholar