Basic–alimentary tractWhole-Genome Analysis and HLA Genotyping of Enteropathy-Type T-Cell Lymphoma Reveals 2 Distinct Lymphoma Subtypes
Section snippets
Morphologic and Clinical Features of ETL Cases
Thirty cases of ETL were selected from the archive of the Lymph Node Reference Center at the Department of Pathology, University of Würzburg, Germany, and from the archive of the Department of Pathology, University of Vienna, Austria. All cases had been classified as ETL based on the criteria defined by the World Health Organization classification of tumors of hematopoietic and lymphoid tissues.10 All cases were reviewed before inclusion in the study. Before the array CGH study, 18 of the 30
Immunohistochemical Analysis of ETL Cases
The immunophenotypical features of the cases are summarized in Table 1. Briefly, the majority of ETL cases (26 of 30) were CD3+/CD4−. Of the remaining 4 cases, 3 were CD3−/CD4− and 1 was CD3−/CD4+. Seventeen ETL cases were CD8+, of which 10 were of monomorphic small- to medium-sized tumor cell morphology. Anaplastic large-cell morphology correlated with CD30 positivity (P = .037) and CD8 negativity (P = .026). Fifteen ETL cases were CD56+, 13 of which were monomorphic, whereas the remaining 2
Discussion
To determine genetic alterations associated with ETL morphology, immunophenotype, and celiac disease–associated HLA genotypes, we studied 30 ETL cases by tiling-path whole-genome array CGH, a molecular technique that allows high-resolution screening of genetic alterations with a resolution of an order of magnitude higher than previous chromosomal CGH and microsatellite analyses.17 Relatively little work has been published on T-cell lymphomas using these new high-resolution techniques. One study
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2022, Clinical Lymphoma, Myeloma and LeukemiaCitation Excerpt :EATL most commonly presents with abdominal pain, malabsorption, diarrhea, weight loss and extra-intestinal spread is rare. TRB or TRG genes are clonally rearranged in almost all cases and most EATLs either show gains of the 9q34 region or deletions of 16q12.1 and HLA-DQ2 haplotype.5 MEITL is an intestinal T-cell lymphoma derived, as like as EATL, from intraepithelial lymphocytes.
Supported by funds from Genome Canada/British Columbia and the Canadian Institute of Health Research; and by the Interdisziplinäres Zentrum für Klinische Forschung, Würzburg, Germany; and by scholarships from the National Sciences and Engineering Council of Canada and the Michael Smith Foundation for Health Research.
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R.J.D. and A.Z. contributed equally to this study.