Employed primers
| Name | Sequence | Tm (°C) | Length (nt) |
| SFB F*† | 5′-AGG AGG AGT CTG CGG CAC ATT AGC-3′ | 62.9 | 24 |
| SFB R*† | 5′-TCC CCA CTG CTG CCT CCC GTA G-3′ | 65.4 | 22 |
| BAC 1114 F‡† | 5′-CGG CAA CGA GCG CAA CCC-3′ | 63.0 | 18 |
| BAC 1275 R‡† | 5′-CCA TTG TAG CAC GTG TGT AGC C-3′ | 58.3 | 22 |
| IL-8 prom F§¶ | 5′-TCA CCA AAT TGT GGA GCT TCA GTA T-3′ | 66.4 | 25 |
| IL-8 prom R§¶ | 5′-GGC TCT TGT CCT AGA AGC TTG TGT-3′ | 65.8 | 24 |
*Sequences for SFB amplification are taken from Suzuki et al26 and Shukla et al27
†Primers were purchased from IDT (IDT Integrated DNA Technologies, Coralville, Iowa, USA).
‡Sequences for total bacteria amplification are taken from Denman and McSweeney28 and Yin et al18
§Sequences for IL-8 genomic DNA amplification are taken from Finotti et al29
¶Primers were purchased from Sigma-Aldrich (Sigma-Aldrich, St.Louis, Missouri, USA).
IL-8, interleukin-8; SFB, segmented filamentous bacteria.