TY - JOUR T1 - Alteration in the gastric microbiota and its restoration by probiotics in patients with functional dyspepsia JF - BMJ Open Gastroenterology DO - 10.1136/bmjgast-2017-000144 VL - 4 IS - 1 SP - e000144 AU - Muneki Igarashi AU - Hirohiko Nakae AU - Takashi Matsuoka AU - Shunsuke Takahashi AU - Takayoshi Hisada AU - Junko Tomita AU - Yasuhiro Koga Y1 - 2017/05/01 UR - http://bmjopengastro.bmj.com//content/4/1/e000144.abstract N2 - Objective The objective of this study was to comparatively analyse the gastric fluid (GF) microbiota between patients with functional dyspepsia (FD) and healthy controls (HC), and to assess the effect of probiotics on the microbiota.Design Twenty-four Japanese patients with FD who met the Rome III definition and 21 age-matched and gender-matched HC volunteers were enrolled. The patients with FD had been treated with LG21, a probiotic strain. The GF was sampled after an overnight fast using a nasogastric tube. The bile acids concentration was determined by ELISA. The V3-V4 region of 16S rRNA gene was amplified using bacterial DNA from the GF, and then about 30 000 high-quality amplicons per sample were grouped into operational taxonomic units for analyses.Results The ratio of GF samples in which the bile acids were detectable was significantly greater in the FD than in the HC groups. In the bacterial composition analysis at the phylum level, the GF microbiota had a Bacteroidetes > Proteobacteria abundance and an absence of Acidobacteria in the FD group, in contrast, the GF microbiota had a Bacteroidetes < Proteobacteria abundance and the presence of Acidobacteria in the HC group. Probiotic therapy in patients with FD shifted the composition of the GF microbiota to that observed in the HC volunteers.Conclusions Alteration in the GF microbiota was found in patients with FD compared with HC volunteers. Reflux of the small intestinal contents, including bile acid and intestinal bacteria, to the stomach was suggested to induce a bacterial composition change and be involved in the pathophysiology underlying FD. Probiotics appear effective in the treatment of FD through the normalisation of gastric microbiota.Trial registration number UMINCTR 000022026; Results. ER -